Download Advances in Photosynthesis Research: Proceedings of the VIth by Dr. Jan M. Anderson (auth.), C. Sybesma (eds.) PDF

By Dr. Jan M. Anderson (auth.), C. Sybesma (eds.)

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Additional info for Advances in Photosynthesis Research: Proceedings of the VIth International Congress on Photosynthesis, Brussels, Belgium, August 1–6, 1983

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We have shown also that LHC-II is an anisotropic membrane-spanning complex, since antibodies against either the purified or delipidated LHC-II induce heavy agglutination when mixed together with RSO or ISO vesicles (Andersson et al. 198la). Recently Ryrie and Anderson (unpublished) combined the more powerful immunoelectrophotetic and Western blot techniques to show that the 26 and 28 kDa peptides of LHC-II each span the membrane. This is true also for the 68, 47 and 41 kDa Chl a-proteins. Our results demonstrate that the specific intrinsic apoproteins that noncovalently bind chlorophylls and carotenoid~ have segments accessible at both the outer and inner membrane surfaces·.

129, 193-196. Dunsmuir P, Smith SM and Bedbrook J (1983) J. Mol. Appl. Genet. in press. Fenna RE and Matthews BW (1977) Brookhaven Symp. Biol. 28, 170-182. Gantt E (1981) Annu. Rev. Plant Physiol. 32, 327-347. 1. 10 Haworth P, Kyle DJ, Horton P and Arntzen CJ (1982) Photochem. Photobiophys. 36, 743-748. Haworth P, Watson JL and Arntzen CJ (1983) Biochim. Biophys. Acta in press. Horton P (1983) FEBS Lett, 152, 47-52. Ish-Shalom D and Ohad I (1983) Biochim. Biophys. Acta 722, 498-507. Jennings RC, Garlaschi FM, Gerola PD and Forti G (1979) Biochim.

1. 7 appressed membranes, Despite membrane fractionation studies indicating that the cyt b/f complex is in both membrane regions, it may be that this complex is located only in stroma-exposed membranes. On membrane shearing it may become uniformlly distributed. This is feasible since no surface-exposed antigenic sites were detected and the cyt b/f complex may be more buried than the other complexes (Morschel, Staehelin 1983). 3. Photosynthetic capacity of thylakoids Chloroplasts with different extents of thylakoid stacking were postulated to have different proportions of thylakoid complexes, hence different photosynthetic yields (Anderson 1982a).

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